Efficient Plastid Transformation in Arabidopsis
نویسندگان
چکیده
منابع مشابه
Efficient Plastid Transformation in Arabidopsis1[OPEN]
Plastid transformation is routine in tobacco (Nicotiana tabacum) but 100-fold less frequent in Arabidopsis (Arabidopsis thaliana), preventing its use in plastid biology. A recent study revealed that null mutations in ACC2, encoding a plastid-targeted acetylcoenzyme A carboxylase, cause hypersensitivity to spectinomycin. We hypothesized that plastid transformation efficiency should increase in t...
متن کاملRecent advances in plastid transformation
Plastid transformation offers a viable alternative to nuclear transformation because of its numerous advantages. It was against this backdrop that various groups of researchers have been exploiting this group of sub-cellular organelles, over the last two decades, for the genetic engineering of agronomic traits and metabolic pathways, as well as for molecular farming for the production of plant-...
متن کاملRegeneration and plastid transformation approaches in Arabidopsis thaliana and Rapid-Cycling Brassica rapa
5 2 Summary Plastid transformation is a well-established tool for biotechnology as it allows high expression levels of proteins and as it provides biological transgene containment because of maternal inheritance of cytoplasmic genes in most crops. In basic research the function and regulation of plastid encoded genes can be further studied with the help of plastid transformation. This study is ...
متن کاملA Major Advance in Plastid Transformation.
In this issue of Plant Physiology, Pal Maliga’s laboratory reported a major advance in plastid transformation. Stable manipulation of the plastid genome of flowering plants was first reported nearly 30 years ago (Svab et al., 1990). The approach was to select transplastomic plants using spectinomycin, which blocks translation by the prokaryotic-type 70S ribosomes found in plastids. Thus, in the...
متن کاملPlastid signals remodel light signaling networks and are essential for efficient chloroplast biogenesis in Arabidopsis.
Plastid signals are among the most potent regulators of genes that encode proteins active in photosynthesis. Plastid signals help coordinate the expression of the nuclear and chloroplast genomes and the expression of genes with the functional state of the chloroplast. Here, we report the isolation of new cryptochrome1 (cry1) alleles from a screen for Arabidopsis thaliana genomes uncoupled mutan...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Physiology
سال: 2017
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.17.00857